Bernard Foley will start at flyhalf for the Wallabies against the Springboks in Perth on Saturday.
Kurtley Beale, who wore the No 10 jersey against the All Blacks in Sydney and Auckland, has been relegated to the bench.
There are two other changes to the starting XV, with Nick Phipps coming in at scrumhalf and James Hanson replacing the injured Nathan Charles at hooker.
'We feel the partnership of Nick and Bernard will best suit the style of game we'll be trying to play,' said coach Ewen McKenzie. 'It's naturally disappointing for the two guys who missed out, but the reality is we have a lot of good players in those playmaking positions.
'We expect them to make an impact playing an important role in how we close out the match.'
Wallabies – 15 Israel Folau, 14 Adam Ashley-Cooper, 13 Tevita Kuridrani, 12 Matt Toomua, 11 Rob Horne, 10 Bernard Foley, 9 Nick Phipps, 8 Wycliff Palu, 7 Michael Hooper, 6 Scott Fardy, 5 Rob Simmons, 4 Sam Carter, 3 James Slipper, 2 James Hanson, 1 Sekope Kepu.
Subs: 16 Saia Fainga'a, 17 Pek Cowan, 18 Ben Alexander, 19 James Horwill, 20 Scott Higginbotham, 21 Matt Hodgson, 22 Nic White, 23 Kurtley Beale.
Photo: Matt King/Getty Images
